Qiaquick multiwell pcr purification handbook acgt, inc. Storage store rneasy minelute spin columns immediately upon receipt at 28c. Under these conditions, these kits can be stored for up to 12 months without showing any reduction in performance and quality. Place a qiaquick spin column in a provided 2 ml collection tube or into a vacuum manifold. Qiaprep miniprep handbook 052004 9 safety information when working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. Strong headwinds cause the glide angle to be steeper due to the slower. Under these conditions, if no expiration date is mentioned on the kit label, qiaquick kits can be stored for at least 12 months without showing any reduction in.
This handbook is intended to serve as a general guide to key legal and select business issues faced by entrepreneurs starting a company to develop universitybased technology. A airplane, b gyroplane, c lighterthanair, d weightshiftcontrol, e glider, and f powered parachute. Department of transportation federal aviation administration 800 independence avenue, sw washington, dc 20591 866 tellfaa 866 8355322. This handbook contains technical information about the qiaquick multiwell pcr purification system to help. Kit contents qiaquick pcr purification kit 50 250 catalog no. Add 1 gel volume of isopropanol to the sample and mix. These are available online in convenient and compact pdf format. Qiaquick gel extraction kit protocol syracuse university. Add deionized water to bring the purified pcr fragments to the original volume. Protocol qiaquick spin handbook 032001 23 qiaquick gel extraction kit protocol using a microcentrifuge this protocol is designed to extract and purify dna of 70 bp qiaquick gel extraction kit for gel the same steps as the manual qg and leads to inefficient dna binding. If you have misplaced your lamotte product instruction or manual you can view and download a pdf of those documents here.
Qiaquick pcr purification kit for purification of up to 10 g pcr products, 100 bp to 10 kb. Read through the qiaquick spin handbook posted at the 7. Protocol qiaquick spin handbook puification kit elution. This kit can also be used for dna cleanup from enzymatic reactions see page 8.
Welcome to the home page of one of the fastest growing departments of biochemistry in the u. Useful when you have a motor or other high currentvoltage load in your circuit. Under these conditions they can be stored for up to 12 months without reduction in performance and. If the direction of the electricity is reversed, the motor will spin in the opposite direction. Up to 400 mg agarose can be processed per spin column. These are available online in convenient and compact pdf.
The lsa category covers a wide variety of aircraft including. Qiaquick spin handbook qiaquick pcr purification kit for purification of pcr products 100 bp 10 kb qiaquick nucleotide removal kit for oligonucleotide 1740mers and dna 40 bp 10 kb cleanup from enzymatic reactions qiaquick gel extraction kit for gel extraction or cleanup of dna 70 bp 10 kb from enzymatic reactions. To bind dna, apply the sample to the qiaquick column and centrifuge for 3060 s. Pcr or other enzymatic reaction or solubilized gel slice. Fully automated purification of nucleic acids or proteins using qiagen spin kits on the qiacube removes the need for. Straightin autorotation a straightin autorotation is one made from altitude with no turns. Qiagen research kits compatible with the qiavac 24 plus application kit accessories plasmid minipreps qiaprep spin miniprep kits dna cleanup qiaquick pcr purification kits qiaquick gel extraction kits qiaquick nucleotide removal kits minelute pcr purification kits. Among the questions addressed in the handbook include. It is important to dry the membrane of the dneasy mini spin column, since residual ethanol may. Dna cleanup using qiacube instruments follows the same steps as the manual procedure i. Place a qiaquick spin column in a provided 2 ml collection tube. Qiaquick spin handbook 032008 19 qiaquick pcr purification kit protocol using a microcentrifuge this protocol is designed to purify single or doublestranded dna fragments from pcr and other enzymatic reactions see page 8. Qiagen qiaquick pcr purification kit 50, for purification of 50 pcr reactions.
Hoffmannla roche owns patents and patent applications pertaining to the application of ninta resin patent series. Dneasy mini spin columns colorless in 2 ml collection tubes 50 250 collection tubes 2 ml 100 500 buffer atl 10 ml 50 ml buffer al 12 ml 54 ml buffer aw1 concentrate 19 ml 95 ml buffer aw2 concentrate ml 66 ml buffer ae 22 ml 2 x 60 ml proteinase k 1. Discard flowthrough and place the qiaquick column back into the same tube. For more information, please refer to the qiaquick spin handbook, march 2008, for technical assistance, please call tollfree 0080022446000, or find regional. Qiaquick spin column, supplied by qiagen, used in various techniques. Protocol qiaquick spin handbook 032001 18 qiaquick pcr purification kit protocol using a microcentrifuge this protocol is designed. Qiacube pure efficiency walkaway spincolumn processing. Under these conditions, qiaquick spin kits can be stored for up to 12 months without showing any reduction in performance and quality.
Fluorescent and radioactive sequencing ligation and transformation restriction amplification labeling in vitro transcription hybridization microinjection table 1. To bind dna, apply the sample to the qiaquick column and centrifuge for 1 min or apply vacuum to the manifold until all the samples have passed through the column. Qiaprep spin miniprep kit protocol using a microcentrifuge. The minelute system combines the convenience of spincolumn technology with the. The international skating union is the international sport federation administering ice skating sports throughout the world. Pdf qiaquick spin handbook 112006 7 qiaquick qiaquick qiaquick pcr purification nucleotide gel extraction kit removal kit kit maximum binding. For more information, please consult the appropriate material safety data sheets msdss.
Handbook 1 1 1 1 1 1 the qiafilter megagiga cartridge is designed for use with a 1 liter, 45 mmneck glass bottle e. The maximum volume of the column reservoir is 800 l. Qiaquick gel extraction kit protocol using a microcentrifuge. University of illinois worchester polytechnic institute. Place the dneasy mini spin column in a new 2 ml collection tube provided, add500. This protocol is designed to purify single or doublestranded dna fragments from pcr and other enzymatic reactions see page 8. Qiaquick spin handbook en print bookmark share pdf 814kb english format file size language download get adobe reader contact qiagen. This list contains instructions and manuals for current products and archived instructions and manuals for discontinued products.
Purification of total dna from animal tissues spin column protocol this protocol is designed for purification of total dna from animal tissues, including rodent tails. Add ethanol 96100%, check cap to see if it has already been added to buffer pe before use see bottle label for volume. For sample volumes of more than 800 l, simply load and spin again. Optimal molar templatetoprimer ratios for manual radioactive sequencing.
Qiaprep miniprep handbook 122006 9 safety information when working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. See qiaquick spin handbook for details on how to set up a vacuum manifold. Qiaquick pcr purification kit 50 from qiagen sample to. The qiaquick system combines the convenience of spincolumn technology with. Qiaquick multiwell pcr purification handbook 092003 5 storage qiaquick 8 and 96 pcr purification kits should be stored dry at room temperature 1525c. Simply search by the product code number using the search or filter tool to the left. We included negative rna samples and template controls in every assay and found all to be negative. Under these conditions they can be stored for up to 12 months without reduction in performance and quality. For longer storage, these kits can also be kept at. Qiaamp minelute virus spin handbook 042010 7 safety information when working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles.
For cleanup of other enzymatic reactions, follow the protocol as described for pcr samples or use the minelute reaction. These are available online in convenient and compact pdf format at. For longer storage, these kits can be stored at 28c. Qiaquick spin handbook 112006 9 qiaquick kits provide high yields of pure nucleic acids, for direct use in applications such as. Qiaquick spin handbook 072002 23 qiaquick gel extraction kit protocol using a microcentrifuge this protocol is designed to extract and purify dna of 70 bp to 10 kb from standard or lowmelt agarose gels in tae or tbe buffer. A detailed protocol for using qiaquick spin columns on the qiacube is provided with. Invert the column and spin it at 270 x g for 2 minutes to collect the sample. Qiaquick spin handbook en print bookmark share pdf 814kb english format file size language. Qiagen guarantees the performance of all products in the manner described in our.
It is important to dry the membrane of the dneasy mini spin. Qiacube follows the same steps as the manual procedure i. Not compatible with disinfecting reagents containing bleach. The rnasefree dnase set, which contains dnase i, buffer rdd and rnasefree water tube, is shipped at ambient temperature. Under these conditions, if no expiration date is mentioned on the kit label, qiaquick kits can be stored for at least 12 months without showing any reduction in performance and quality. For longer storage, qiaquick spin kits can also be stored at 28c, but in this case the buffers should be redissolved before use. To bind dna, apply the sample to the qiaquick column, and centrifuge for 1 min. Qiaquick spin columns can now be used on any vacuum manifold with luer connectors. Qiaquick spin columns, supplied by qiagen, used in various techniques. This protocol is designed for purification of up to 20 g of highcopy plasmid dna from. Qiaquick pcr purification kit protocol using a vacuum manifold. Qiaquick spin handbook 032008 9 qiaquick kits provide high yields of pure nucleic acids, for direct use in applications such as. Purification of total dna from animal tissues spin. Qiaquick spin kits should be stored dry at room temperature 1525c.
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